MAOB Monoklonaler Antikörper

MAOB Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B12F3

Kat-Nr. : 66107-1-PBS

Synonyme

2B12F3, Amine oxidase [flavin-containing] B, EC:1.4.3.21, MAO B, MAO-B

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  WB analysis of fetal human brain using 66107-1-Ig (same clone as 66107-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66107-1-Ig (same clone as 66107-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66107-1-Ig (same clone as 66107-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 66107-1-Ig (same clone as 66107-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 66107-1-Ig (same clone as 66107-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66107-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA MAOB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAOB fusion protein Ag17918
• Vollständiger Name: monoamine oxidase B
• Berechnetes Molekulargewicht: 520 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC022494
• Gene symbol: MAOB
• Gene ID (NCBI): 4129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position(PMID:3387449).