MAP1B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MAP1B Polyklonal Antikörper für FC, IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Rezensionen (1)

Kat-Nr. : 21633-1-AP

Synonyme

MAP 1B, MAP1B, MAP5

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse cerebellum using 21633-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21633-1-AP (MAP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21633-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21633-1-AP (MAP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 21633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21633-1-AP (MAP1B antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21633-1-AP (MAP1B antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21633-1-AP (MAP1B antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21633-1-AP (MAP1B antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 21633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21633-1-AP (MAP1B antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 21633-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 21633-1-AP (MAP1B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 21633-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 21633-1-AP (MAP1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 21633-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug MAP1B antibody (21633-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen, Maushirngewebe
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IF: See 3 publications below

Produktinformation

21633-1-AP bindet in WB, IHC, IF, FC, ELISA MAP1B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAP1B fusion protein Ag16255
• Vollständiger Name: microtubule-associated protein 1B
• Berechnetes Molekulargewicht: 2468 aa, 271 kDa
• Beobachtetes Molekulargewicht: 320 kDa
• GenBank-Zugangsnummer: BC141853
• Gene symbol: MAP1B
• Gene ID (NCBI): 4131
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)

Publikationen

Species	Application	Title
human	WB	EMBO Rep; MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules.; Authors - Kohei Arasaki ; KD Validated
mouse	WB	Cereb Cortex; eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures.; Authors - Christopher Heise
mouse	IF	Front Cell Neurosci; Endothelin-1, over-expressed in SOD1G93A mice, aggravates injury of NSC34-hSOD1G93A cells through complicated molecular mechanism revealed by quantitative proteomics analysis; Authors - Yingzhen Zhang
mouse	WB	Hum Mol Genet; Microtubule associated protein 1B dysregulates microtubule dynamics and neuronal mitochondrial transport in Spinal Muscular Atrophy.; Authors - Gamze Bora
human	WB	Cell Cycle; Depletion of JMJD5 sensitizes tumor cells to microtubule-destabilizing agents by altering microtubule stability.; Authors - Junyu Wu
mouse	WB,IF	Neurochem Int; FMRP-absence-induced up-regulation of hypothalamic MAP1B expression decreases AgRP level linking with reduces in food intake and body weight.; Authors - Jing-Yi Long ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Albane (Verified Customer) (02-18-2020)

A lot of bands, none at the correct size

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: SH-SY5Y

MAP1B Antibody Western Blot, validation (1/1000 dilution) in SH-SY5Y (Cat no:21633-1-AP)

Verwandte Produkte

GABRR1 Polyclonal antibody

25899-1-AP

GSK3B Polyclonal antibody

22104-1-AP

JNK Polyclonal antibody

24164-1-AP

MST1 Polyclonal antibody

22245-1-AP