MAP1B Monoklonaler Antikörper

MAP1B Monoklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E6B9

Kat-Nr. : 67423-1-PBS

Synonyme

1E6B9, MAP1 light chain LC1, MAP-1B, MAP1B heavy chain, Microtubule-associated protein 1B

---

• 

  IHC staining of rat brain using 67423-1-Ig (same clone as 67423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67423-1-Ig (same clone as 67423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67423-1-Ig (same clone as 67423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67423-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 67423-1-Ig (same clone as 67423-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 67423-1-Ig (MAP1B antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67423-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67423-1-PBS bindet in IHC, IF-P, ELISA MAP1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAP1B fusion protein Ag17709
• Vollständiger Name: microtubule-associated protein 1B
• Berechnetes Molekulargewicht: 2468 aa, 271 kDa
• GenBank-Zugangsnummer: BC141853
• Gene symbol: MAP1B
• Gene ID (NCBI): 4131
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)