LC3 Monoklonaler Antikörper

LC3 Monoklonal Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A5E4

Kat-Nr. : 66139-3-Ig

Synonyme

MAP1LC3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3A, MAP1 light chain 3-like protein 1

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  IF Staining of NIH/3T3 using 66139-3-Ig

  Immunofluorescent analysis of (4% PFA) fixed Chloroquine treated NIH/3T3 cells using MAP1LC3A,LC3 antibody (66139-3-Ig, Clone: 2A5E4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HeLa using 66139-3-Ig

  Immunofluorescent analysis of (4% PFA) fixed Chloroquine treated HeLa cells using MAP1LC3A,LC3 antibody (66139-3-Ig, Clone: 2A5E4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of PC-12 using 66139-3-Ig

  Immunofluorescent analysis of (4% PFA) fixed Chloroquine treated PC-12 cells using MAP1LC3A,LC3 antibody (66139-3-Ig, Clone: 2A5E4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Chloroquine treated NIH/3T3 cells, mit Chloroquin behandelte HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66139-3-Ig bindet in IF/ICC, ELISA LC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LC3 fusion protein Ag17959
• Vollständiger Name: microtubule-associated protein 1 light chain 3 alpha
• Berechnetes Molekulargewicht: 121 aa, 14 kDa
• GenBank-Zugangsnummer: BC015810
• Gene symbol: LC3
• Gene ID (NCBI): 84557
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LC3A, also named as MAP1LC3A, LC3, MAP1ALC3 and MAP1BLC3, belongs to the MAP1 LC3 family. LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B, which are microtubule-associated proteins that mediate the physical interactions between microtubules and components of the cytoskeleton. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. (PMID:11060023) MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form.(PMID:12740394 )