MAP2 Rekombinanter Antikörper

MAP2 Rekombinant Antikörper für WB, IHC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

CloneNo.

241653E9

Kat-Nr. : 84306-3-RR

Synonyme

241653E9, MAP 2, MAP-2, MAP2A, MAP2B

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  WB analysis using 84306-3-RR

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84306-3-RR (MAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84306-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84306-3-RR (MAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 84306-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84306-3-RR (MAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 84306-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84306-3-RR (MAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 84306-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6, green) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).MAP2 antibody (84306-3-RR, Clone: 241653E9,red ) at dilution of 1:210 and FlexAble CoraLite® Plus 594 Antibody Labeling Kit for Rabbit IgG(KFA009). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 84306-3-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody (84306-3-RR, Clone: 241653E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of rat brain using 84306-3-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using MAP2 antibody (84306-3-RR, Clone: 241653E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 GFAP antibody (CL594-16825, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe
• Erfolgreiche Detektion in IF-Fro: Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)-P: IF-P : 1:125-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84306-3-RR bindet in WB, IHC, IF-P, IF-Fro, ELISA MAP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MAP2 fusion protein Ag11580
• Vollständiger Name: microtubule-associated protein 2
• Berechnetes Molekulargewicht: 200 kDa
• Beobachtetes Molekulargewicht: 280-300 kDa, 70-85 kDa
• GenBank-Zugangsnummer: BC038857
• Gene symbol: MAP2
• Gene ID (NCBI): 4133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in the brain and has an important role in neuronal morphogenesis. Multiple high MW and low MW MAP2 isoforms are expressed within the proximal segment of axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally-specific manner. The 280 kDa MAP2B is present throughout rat brain development, and the slightly larger MAP2A appears first during the end of the second week of postnatal life. MAP2C, composed of several bands of about 70 kDa, is present during early brain development and largely disappears from the mature brain except for the retina, olfactory bulb, and cerebellum. In addition, some isoforms with lower MW around 50-60 kDa also exist. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.