MAP2 Rekombinanter Antikörper

MAP2 Rekombinant Antikörper für WB, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241653H8

Kat-Nr. : 84306-4-PBS

Synonyme

241653H8, MAP 2, MAP-2, MAP2A, MAP2B

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  WB analysis using 84306-4-RR (same clone as 84306-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84306-4-RR (MAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84306-4-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 84306-4-RR (same clone as 84306-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using MAP2 antibody (84306-4-RR, Clone: 241653H8,red ) at dilution of 1:210 and FlexAble CoraLite® Plus 594 Antibody Labeling Kit for Rabbit IgG(KFA009), GFAP antibody (81063-1-RR, Clone: 4C6, green) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84306-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 84306-4-RR (same clone as 84306-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MAP2 antibody (84306-4-RR, Clone: 241653H8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84306-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 84306-4-RR (same clone as 84306-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody (84306-4-RR, Clone: 241653H8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84306-4-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 84306-4-RR (same clone as 84306-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using MAP2 antibody (84306-4-RR, Clone: 241653H8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red). This data was developed using the same antibody clone with 84306-4-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 84306-4-RR (same clone as 84306-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using MAP2 antibody (84306-4-RR, Clone: 241653H8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84306-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84306-4-PBS bindet in WB, IF/ICC, IF-P, IF-Fro, Indirect ELISA MAP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MAP2 fusion protein Ag11580
• Vollständiger Name: microtubule-associated protein 2
• Berechnetes Molekulargewicht: 200 kDa
• Beobachtetes Molekulargewicht: 280-300 kDa, 70-85 kDa
• GenBank-Zugangsnummer: BC038857
• Gene symbol: MAP2
• Gene ID (NCBI): 4133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in the brain and has an important role in neuronal morphogenesis. Multiple high MW and low MW MAP2 isoforms are expressed within the proximal segment of axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally-specific manner. The 280 kDa MAP2B is present throughout rat brain development, and the slightly larger MAP2A appears first during the end of the second week of postnatal life. MAP2C, composed of several bands of about 70 kDa, is present during early brain development and largely disappears from the mature brain except for the retina, olfactory bulb, and cerebellum. In addition, some isoforms with lower MW around 50-60 kDa also exist. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.