MEK1/2 Monoklonaler Antikörper

MEK1/2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

CloneNo.

1H8C2

Kat-Nr. : 67410-1-Ig

Synonyme

MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2

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  WB analysis using 67410-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67410-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67410-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67410-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MEK1/2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67410-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MEK1/2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67410-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEK1/2 antibody (67410-1-Ig, Clone: 1H8C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, MOLT-4-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 1 publications below

Produktinformation

67410-1-Ig bindet in WB, IHC, IF/ICC, ELISA MEK1/2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MEK1/2 fusion protein Ag29871
• Vollständiger Name: mitogen-activated protein kinase kinase 2
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44-47 kDa
• GenBank-Zugangsnummer: BC018645
• Gene symbol: MEK2
• Gene ID (NCBI): 5605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 67410-1-Ig can recognize both the MEK2 and MEK1.

Publikationen

Species	Application	Title
mouse	IHC	Front Immunol; Development and validation of cuproptosis-related lncRNAs associated with pancreatic cancer immune microenvironment based on single-cell; Authors - Yimeng Sun
mouse	WB	Mol Cell Biochem; Anti-inflammatory effects of extracellular vesicles from Morchella on LPS-stimulated RAW264.7 cells via the ROS-mediated p38 MAPK signaling pathway.; Authors - Qi Chen
human	WB	PNAS Nexus; A potent tumor-selective ERK pathway inactivator with high therapeutic index.; Authors - Zehua Zuo
human	WB	Signal Transduct Target Ther; IGF-1R inhibition induces MEK phosphorylation to promote survival in colon carcinomas.; Authors - Qing Wang
mouse	WB	Proc Natl Acad Sci U S A; ERK and c-Myc signaling in host-derived tumor endothelial cells is essential for solid tumor growth; Authors - Zehua Zuo
human	WB	Front Med; 5'-tiRNA-Gln inhibits hepatocellular carcinoma progression by repressing translation through the interaction with eukaryotic initiation factor 4A-I; Authors - Chengdong Wu