MAP2K3 Polyklonaler Antikörper

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• KD/KO Validated

MAP2K3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 13898-1-AP

Synonyme

MEK3, MAPKK3, MAPKK 3, MAPK/ERK kinase 3, MAP kinase kinase 3

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  WB analysis using 13898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13898-1-AP (MAP2K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13898-1-AP (MAP2K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below

Produktinformation

13898-1-AP bindet in WB, ELISA MAP2K3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAP2K3 fusion protein Ag4861
• Vollständiger Name: mitogen-activated protein kinase kinase 3
• Berechnetes Molekulargewicht: 347 aa, 39 kDa
• Beobachtetes Molekulargewicht: 36-40 kDa
• GenBank-Zugangsnummer: BC032478
• Gene symbol: MAP2K3
• Gene ID (NCBI): 5606
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP2K3, also named MKK3, is a member of the dual specificity kinase group and serves as a specific activator of p38 MAPK with MKK6. MKK3 can be phosphorylated by cytokines and environmental stress at sites Ser189 and Thr193 by MKKK proteins (MEKK 1-4). MKK3 plays a key role in cell differentiation, motility, division, and death. It has been reported that MKK3 targeting may represent an effective and potentially safe therapeutic strategy to selectively kill cancer cells in colorectal cancer patients. (PMID: 31695024, 30770795, 26805700, 8622669)

Publikationen

Species	Application	Title
human	WB	Biomed Pharmacother; Identification of MAP Kinase Kinase 3 as a protein target of myricetin in non-small cell lung cancer cells; Authors - Ling Gu ; KD Validated
human	WB	Cell Mol Life Sci; HERC2 deficiency activates C-RAF/MKK3/p38 signalling pathway altering the cellular response to oxidative stress; Authors - Joan Sala-Gaston
mouse	WB	Food Funct; L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.; Authors - Kehong Liu
human	WB	Sci Rep; The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling.; Authors - Leonardo Pedrazza ; KD Validated
mouse	WB	Mol Immunol; Spiroplasma eriocheiris induces mouse 3T6-Swiss albino cell apoptosis that associated with the infection mechanism.; Authors - Libo Hou
human	WB	PLoS One; Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.; Authors - Xu Jiang