MAP2K3 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

MAP2K3 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3O10

Kat-Nr. : 80137-1-PBS

Synonyme

MEK3, MAPKK 3, MAPK/ERK kinase 3, MAP kinase kinase 3, EC:2.7.12.2

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  WB analysis using 80137-1-RR (same clone as 80137-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 80137-1-RR (same clone as 80137-1-PBS)

  WB result of MAP2K3 antibody (80137-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP2K3 transfected HepG2 cells. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 80137-1-RR (same clone as 80137-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80137-1-RR (same clone as 80137-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80137-1-RR (same clone as 80137-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-1080 using 80137-1-RR (same clone as 80137-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

• 

  WB analysis of zebrafish using 80137-1-RR (same clone as 80137-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 80137-1-RR (same clone as 80137-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP2K3 antibody (80137-1-RR, Clone: 3O10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue) This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80137-1-PBS bindet in WB, IF/ICC, Indirect ELISA MAP2K3 und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase 3
• Berechnetes Molekulargewicht: 347 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC032478
• Gene symbol: MAP2K3
• Gene ID (NCBI): 5606
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP2K3, also named MKK3, is a member of dual specificity kinase group and serves as a specific activator of p38 MAPK with MKK6. MKK3 can be phosphorylated by cytokines and environmental stress at sites Ser189 and Thr193 by MKKK proteins (MEKK 1-4). MKK3 plays a key role in cell differentiation, motility, division and death. It has been reported that MKK3 targeting may represent an effective and potentially safe therapeutic strategy to selectively kill cancer cells in colorectal cancer patients. (PMID: 31695024, 30770795, 26805700, 8622669)