MAP2K3 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

MAP2K3 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3O10

Kat-Nr. : 80137-1-RR

Synonyme

MEK3, MAPKK 3, MAPK/ERK kinase 3, MAP kinase kinase 3, EC:2.7.12.2

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  WB analysis using 80137-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 80137-1-RR

  WB result of MAP2K3 antibody (80137-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP2K3 transfected HepG2 cells.

• 

  WB analysis using 80137-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 80137-1-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80137-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 80137-1-RR

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of zebrafish using 80137-1-RR

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 80137-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP2K3 antibody (80137-1-RR, Clone: 3O10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue)

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, A431-Zellen, C2C12-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HT-1080.Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Zebrafischgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

80137-1-RR bindet in WB, IF/ICC, ELISA MAP2K3 und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase 3
• Berechnetes Molekulargewicht: 347 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC032478
• Gene symbol: MAP2K3
• Gene ID (NCBI): 5606
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP2K3, also named MKK3, is a member of dual specificity kinase group and serves as a specific activator of p38 MAPK with MKK6. MKK3 can be phosphorylated by cytokines and environmental stress at sites Ser189 and Thr193 by MKKK proteins (MEKK 1-4). MKK3 plays a key role in cell differentiation, motility, division and death. It has been reported that MKK3 targeting may represent an effective and potentially safe therapeutic strategy to selectively kill cancer cells in colorectal cancer patients. (PMID: 31695024, 30770795, 26805700, 8622669)

Publikationen

Species	Application	Title
mouse	WB	Acta Pharm Sin B; Reversing metabolic reprogramming by CPT1 inhibition with etomoxir promotes cardiomyocyte proliferation and heart regeneration via DUSP1 ADP-ribosylation-mediated p38 MAPK phosphorylation; Authors - Luxun Tang