MAP2K4 Polyklonaler Antikörper

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• KD/KO Validated

MAP2K4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 17340-1-AP

Synonyme

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

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Galerie der Validierungsdaten

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  WB analysis using 17340-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17340-1-AP (MAP2K4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17340-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17340-1-AP (MAP2K4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 17340-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 17340-1-AP (MAP2K4 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17340-1-AP (MAP2K4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17340-1-AP (MAP2K4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17340-1-AP (MAP2K4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17340-1-AP (MAP2K4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17340-1-AP (MAP2K4 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17340-1-AP (MAP2K4 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 2 publications below

Produktinformation

17340-1-AP bindet in WB, IHC, ELISA MAP2K4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAP2K4 fusion protein Ag11376
• Vollständiger Name: mitogen-activated protein kinase kinase 4
• Berechnetes Molekulargewicht: 399 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44-50 kDa
• GenBank-Zugangsnummer: BC036032
• Gene symbol: MAP2K4
• Gene ID (NCBI): 6416
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP2K4 is also named as JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MAP2K4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB,IHC	EBioMedicine; PMID: 31594754; Authors - Yiyi Liu
mouse,rat	WB	J Hazard Mater; Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.; Authors - Junrong Wu
mouse	WB	J Cell Mol Med; NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection.; Authors - Ze-Qing Pu
rat	WB	Cells; Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning.; Authors - Elaheh Soleimanpour
human	WB	Int J Biochem Cell Biol; Androgen-induced miR-27A acted as a tumor suppressor by targeting MAP2K4 and mediated prostate cancer progression.; Authors - Xuechao Wan ; KD Validated
human	WB	Dig Dis Sci; LPS Induced miR-181a Promotes Pancreatic Cancer Cell Migration via Targeting PTEN and MAP2K4.; Authors - Jianhui Liu