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MAP2K4 Monoklonaler Antikörper

MAP2K4 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E4C1

Kat-Nr. : 67333-1-PBS

Synonyme

MEK4, 2E4C1, c-Jun N-terminal kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 4, EC:2.7.12.2

Filter:
  • Western Blot (WB) analysis of various lysates using MAP2K4 Monoclonal antibody (67333-1-Ig)

    WB analysis using 67333-1-Ig (same clone as 67333-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67333-1-Ig (MAP2K4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAP2K4 Monoclonal antibody (67333-1-Ig)

    IHC staining of human breast cancer using 67333-1-Ig (same clone as 67333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAP2K4 Monoclonal antibody (67333-1-Ig)

    IHC staining of human breast cancer using 67333-1-Ig (same clone as 67333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAP2K4 Monoclonal antibody (67333-1-Ig)

    IF Staining of HepG2 using 67333-1-Ig (same clone as 67333-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAP2K4 antibody (67333-1-Ig, Clone: 2E4C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67333-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MAP2K4 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen MAP2K4 fusion protein Ag11348
Vollständiger Name mitogen-activated protein kinase kinase 4
Berechnetes Molekulargewicht 399 aa, 44 kDa
Beobachtetes Molekulargewicht44-50 kDa
GenBank-ZugangsnummerBC036032
Gene symbol MAP2K4
Gene ID (NCBI) 6416
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.