MEK5 Polyklonaler Antikörper

MEK5 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 15758-1-AP

Synonyme

MAP2K5, MKK5, MAPKK5, HsT17454, EC:2.7.12.2

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  WB analysis of HEK-293T using 15758-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15758-1-AP (MEK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15758-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15758-1-AP (MEK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 15758-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15758-1-AP (MEK5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 15758-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 15758-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 15758-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEK5 antibody (15758-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, A431-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

15758-1-AP bindet in WB, IHC, IF/ICC, ELISA MEK5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MEK5 fusion protein Ag8351
• Vollständiger Name: mitogen-activated protein kinase kinase 5
• Berechnetes Molekulargewicht: 448 aa, 50 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC008838
• Gene symbol: MEK5
• Gene ID (NCBI): 5607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Int J Hyperthermia; Decrease in MAP3Ks expression enhances the cell death caused by hyperthermia.; Authors - Atsushi Enomoto
rat	WB	Pharm Biol; Injection of YiQiFuMai powder protects against heart failure via inhibiting p38 and ERK1/2 MAPKs activation.; Authors - Yongwei Nie