MEK6 Polyklonaler Antikörper

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• KD/KO Validated

MEK6 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 12745-1-AP

Synonyme

MAP2K6, MKK6, Dual specificity mitogen-activated protein kinase kinase 6, EC:2.7.12.2, MAP kinase kinase 6

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  WB analysis of HeLa using 12745-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12745-1-AP (MEK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12745-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12745-1-AP (MEK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12745-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12745-1-AP (MEK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12745-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12745-1-AP (MEK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Nierengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Produktinformation

12745-1-AP bindet in WB, IHC, IF, ELISA MEK6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MEK6 fusion protein Ag3706
• Vollständiger Name: mitogen-activated protein kinase kinase 6
• Berechnetes Molekulargewicht: 334 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC012009
• Gene symbol: MEK6
• Gene ID (NCBI): 5608
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAPKK6 (Dual specificity mitogen activated protein kinase kinase 6), also known as MAP2K6 / MEK6 / MKK6, is a member of MAPKK protein kinase family. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase. MEK6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. MEK6 plays a complex role in cancer, with some evidence showing it can promote tumor growth. Overexpression of MEK6 has been linked to decreased survival in lung adenocarcinoma, breast cancer, and melanoma.

Publikationen

Species	Application	Title
mouse	WB	Food Funct; L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.; Authors - Kehong Liu
human	WB	Sci Rep; The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling.; Authors - Leonardo Pedrazza
	WB	Nat Aging; Skin chronological aging drives age-related bone loss via secretion of cystatin-A; Authors - Wenquan Liang
human	WB	Acta Pharmacol Sin; Targeting PRSS23 with tipranavir induces gastric cancer stem cell apoptosis and inhibits growth of gastric cancer via the MKK3/p38 MAPK-IL24 pathway; Authors - Ji-Xian Xiong ; KD Validated
human	WB	Cell Cycle; SAMHD1, positively regulated by KLF4, suppresses the proliferation of gastric cancer cells through MAPK p38 signaling pathway; Authors - Zhangming Chen
human	WB	Sci Rep; Identification of a novel immune checkpoint-related gene signature predicts prognosis and immunotherapy in breast cancer and experiment verification; Authors - Ke Yin