MAP3K1 Polyklonaler Antikörper

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• KD/KO Validated

MAP3K1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19970-1-PBS

Synonyme

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  WB analysis using 19970-1-AP (same clone as 19970-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19970-1-AP (same clone as 19970-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 19970-1-AP (same clone as 19970-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 19970-1-AP (same clone as 19970-1-PBS)

  IP result of anti-MAP3K1 (IP:19970-1-AP, 4ug; Detection:19970-1-AP 1:1000) with NIH/3T3 cells lysate 1120 ug. This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 19970-1-AP (same clone as 19970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19970-1-AP (MAP3K1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 19970-1-AP (same clone as 19970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19970-1-AP (MAP3K1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19970-1-AP (same clone as 19970-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19970-1-AP (MAP3K1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19970-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MAP3K1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 1
• Berechnetes Molekulargewicht: 164 kDa
• Beobachtetes Molekulargewicht: 164 kDa
• GenBank-Zugangsnummer: NM_005921
• Gene symbol: MAP3K1
• Gene ID (NCBI): 4214
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP3K1, also named as MAPKKK1, MEKK and MEKK1, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K1 is a component of a protein kinase signal transduction cascade. MAP3K1 activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. It activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. It catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes the N-term of MAP3K1.