TAK1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TAK1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(55)

Kat-Nr. : 12330-2-AP

Synonyme

MAP3K7, EC:2.7.11.25, MAP3K 7, mitogen activated protein kinase kinase kinase 7, Mitogen-activated protein kinase kinase kinase 7

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  WB analysis of NIH/3T3 using 12330-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12330-2-AP

  WB result of TAK1 antibody (12330-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAK1 transfected NIH/3T3 cells.

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  WB analysis of A375 using 12330-2-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 12330-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 12330-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12330-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12330-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of CHO using 12330-2-AP

  CHO cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12330-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12330-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12330-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 12330-2-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 12330-2-AP

  IP result of anti-TAK1 (IP:12330-2-AP, 4ug; Detection:12330-2-AP 1:1000) with NIH/3T3 cells lysate 1200 ug.

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  IHC staining of human prostate cancer using 12330-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12330-2-AP (TAK1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12330-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12330-2-AP (TAK1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12330-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12330-2-AP (TAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12330-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12330-2-AP (TAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12330-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12330-2-AP (TAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12330-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12330-2-AP (TAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 12330-2-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using TAK1 antibody (12330-2-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, A2780-Zellen, A375-Zellen, A431-Zellen, CHO-Zellen, COLO 320-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen, Mausherzgewebe, SKOV-3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 51 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 4 publications below
• CoIP: See 3 publications below

Produktinformation

12330-2-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA TAK1 und zeigt Reaktivität mit hamster, human, Maus

• Getestete Reaktivität: hamster, human, Maus
• In Publikationen genannte Reaktivität: human, Fisch, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAK1 fusion protein Ag3041
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 7
• Berechnetes Molekulargewicht: 579 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64-67 kDa, 75-80 kDa
• GenBank-Zugangsnummer: BC017715
• Gene symbol: TAK1
• Gene ID (NCBI): 6885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transforming growth factor-β (TGF-β)-activated kinase 1 (TAK1), also known as MAP3K7-mitogen-activated protein kinase kinase kinase 7-  is a serine/threonine  protein kinase triggering phosphorylation of AMP-activated protein kinase (AMPK) and playing a role in the signal transduction of TGF-β, bone morphogenetic  proteins (BMPs), and various proinflammatory mediators.

What is the molecular weight of TAK1? Are there any isoforms of TAK1?

The molecular weight of full-length TAK1 is 55 kDa. There are four splicing variants of TAK1. TAK1-B represents the full-length variant, while TAK1-A, TAK1-C, and TAK1-D lack exons 12 and/or 16. TAK-1 variants differ in their expression levels across tissues (PMID: 11118615).

Is TAK1 post-translationally modified?

TAK1 kinase activity is modulated by interacting with TAK1-binding proteins (TABs). Binding  to  TABs  triggers autophosphorylation of TAK1 and depends on  polyubiquitination of receptor-interacting protein 1 (RIP1) (PMID: 8638164 and 11460167). Additionally, TAK1 can be ubiquitinated, acetylated, and O-glycosylated, which impacts its activity (PMID: 28106845).

What is the subcellular localization of TAK1?

TAK1 is present in the cytoplasm.

What is the tissue expression pattern of TAK1?

TAK1 is ubiquitously expressed.

How can TAK1 activity be monitored?

TAK1 requires phosphorylation for its activity. TAK1 autophosphorylation in the presence of TBPs occurs on Thr178, Thr184, Thr187, and Ser192 residues. The most commonly used phosphorylation readout is Thr187 phosphorylation.

Publikationen

Species	Application	Title
mouse	WB	Blood; Platelet NLRP6 protects against microvascular thrombosis in sepsis; Authors - Huimin Jiang
mouse	WB	J Adv Res; Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DDX5-ATF1-PPP2R5A signaling; Authors - Di Fan
human	WB	EMBO J; CARS senses cysteine deprivation to activate AMPK for cell survival.; Authors - Mengqiu Yuan ; KD Validated
mouse	WB	Cell Death Differ; Protein phosphatase 6 is a key factor regulating spermatogenesis.; Authors - Wen-Long Lei
mouse	WB	Diabetes; Hepatic P38 Activation Modulates Systemic Metabolism Through Fgf21-Mediated Interorgan Communication.; Authors - Wei Liu
human	WB	EMBO Rep; Mycobacterium tuberculosis protein kinase G acts as an unusual ubiquitinating enzyme to impair host immunity.; Authors - Jing Wang