MAP4K2 Polyklonaler Antikörper

MAP4K2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55244-1-AP

Synonyme

BL44, GC kinase, GCK, Germinal center kinase, MAP4K2, MEK kinase kinase 2, MEKKK 2, Rab8 interacting protein, RAB8IP

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 55244-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55244-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 55244-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 55244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 55244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 55244-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 55244-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55244-1-AP bindet in WB, IHC, ELISA MAP4K2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase kinase 2
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 85-91 kDa, 58 kDa
• GenBank-Zugangsnummer: NM_004579
• Gene symbol: MAP4K2
• Gene ID (NCBI): 5871
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP4K2,also named as GCK and RAB8IP, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. MAP4K2 may play a role in the regulation of vesicle targeting or fusion. This antibody is specific to MAP4K2.