MAP4K2 Polyklonaler Antikörper

MAP4K2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55244-1-PBS

Synonyme

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  WB analysis of mouse brain using 55244-1-AP (same clone as 55244-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 55244-1-AP (same clone as 55244-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 55244-1-AP (same clone as 55244-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 55244-1-AP (same clone as 55244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 55244-1-AP (same clone as 55244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 55244-1-AP (same clone as 55244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 55244-1-AP (same clone as 55244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55244-1-PBS bindet in WB, IHC, Indirect ELISA MAP4K2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase kinase 2
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 85-91 kDa, 58 kDa
• GenBank-Zugangsnummer: NM_004579
• Gene symbol: MAP4K2
• Gene ID (NCBI): 5871
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP4K2,also named as GCK and RAB8IP, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. MAP4K2 may play a role in the regulation of vesicle targeting or fusion. This antibody is specific to MAP4K2.