MAP4K3 Polyklonaler Antikörper

MAP4K3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30716-1-PBS

Synonyme

MEKKK 3, MEK kinase kinase 3, MAPKKKK3, GLK, Germinal center kinase-related protein kinase

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  WB analysis using 30716-1-AP (same clone as 30716-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30716-1-AP (MAP4K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30716-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 30716-1-AP (same clone as 30716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30716-1-AP (MAP4K3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30716-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 30716-1-AP (same clone as 30716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30716-1-AP (MAP4K3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30716-1-PBS in a different storage buffer formulation.

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  IF Staining of U-87 MG using 30716-1-AP (same clone as 30716-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using MAP4K3 antibody (30716-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30716-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30716-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MAP4K3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAP4K3 fusion protein Ag33512
• Vollständiger Name: mitogen-activated protein kinase kinase kinase kinase 3
• Berechnetes Molekulargewicht: 101 kDa
• Beobachtetes Molekulargewicht: 99-101 kDa
• GenBank-Zugangsnummer: BC071579
• Gene symbol: MAP4K3
• Gene ID (NCBI): 8491
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP4K3(Mitogen-activated protein kinase kinase kinase kinase 3) has kinase activity and that it activates JNK but not MAPK1, MAPK3 or MAPK14 through the activation of MAP3K1 and MAP2K4. Endogenous MAP4K3 kinase activity could be stimulated by exposure to ultraviolet radiation or to tumor necrosis factor.