MAP4K4 Polyklonaler Antikörper

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• KD/KO Validated

MAP4K4 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55247-1-PBS

Synonyme

EC:2.7.11.1, FLH21957, HGK, HPK/GCK like kinase HGK, HPK/GCK-like kinase HGK

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  WB analysis of Jurkat using 55247-1-AP (same clone as 55247-1-PBS)

  WB result of MAP4K4 antibody (55247-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP4K4 transfected Jurkat cells. This data was developed using the same antibody clone with 55247-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 55247-1-AP (same clone as 55247-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55247-1-AP (MAP4K4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55247-1-PBS in a different storage buffer formulation.

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  WB analysis using 55247-1-AP (same clone as 55247-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55247-1-AP (MAP4K4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55247-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 55247-1-AP (same clone as 55247-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55247-1-AP (MAP4K4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55247-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 55247-1-AP (same clone as 55247-1-PBS)

  IP result of anti-MAP4K4 (IP:55247-1-AP, 4ug; Detection:55247-1-AP 1:300) with Jurkat cells lysate 4000 ug. This data was developed using the same antibody clone with 55247-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 55247-1-AP (same clone as 55247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55247-1-AP (MAP4K4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55247-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 55247-1-AP (same clone as 55247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55247-1-AP (MAP4K4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55247-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 55247-1-AP (same clone as 55247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55247-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 55247-1-AP (same clone as 55247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55247-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 55247-1-AP (same clone as 55247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55247-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 55247-1-AP (same clone as 55247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55247-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55247-1-PBS bindet in WB, IHC, IP, Indirect ELISA MAP4K4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase kinase 4
• Berechnetes Molekulargewicht: 151 kDa
• Beobachtetes Molekulargewicht: 130-160 kDa
• GenBank-Zugangsnummer: NM_004834
• Gene symbol: MAP4K4
• Gene ID (NCBI): 9448
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP4K4, also named as RAB8IPL1, GLK and MEKKK4, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It may play a role in the response to environmental stress. MAP4K4 appears to act upstream of the JUN N-terminal pathway. This antibody is specific to MAP4K4.