MAP6/STOP Polyklonaler Antikörper

MAP6/STOP Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25717-1-PBS

Synonyme

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  WB analysis using 25717-1-AP (same clone as 25717-1-PBS)

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 25717-1-AP (MAP6/STOP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

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  WB analysis using 25717-1-AP (same clone as 25717-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25717-1-AP (MAP6/STOP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 25717-1-AP (same clone as 25717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25717-1-AP (MAP6/STOP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 25717-1-AP (same clone as 25717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25717-1-AP (MAP6/STOP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 25717-1-AP (same clone as 25717-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using MAP6/STOP antibody (25717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25717-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25717-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MAP6/STOP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAP6/STOP fusion protein Ag22471
• Vollständiger Name: microtubule-associated protein 6
• Berechnetes Molekulargewicht: 813 aa, 87 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC139780
• Gene symbol: MAP6
• Gene ID (NCBI): 4135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP6, also named STOP protein, has been isolated from brain preparations but is also present in other tissues such as the skeletal muscles and heart. MAP6 is a calmodulin-binding and calmodulin-regulated protein that is involved in microtubule stabilization. Different isoforms of STOP protein arise from alternative mRNA splicing and transcription initiation sites using a single gene. The neuronal MAP6 isoforms N-STOP (120 kDa) and E-STOP (80 kDa), the astrocyte MAP6 isoform A-STOP (60 KDa), and a fibroblastic MAP6 isoform F-STOP (48 kDa) have been identified.