MAP7 Polyklonaler Antikörper

MAP7 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13446-1-PBS

Synonyme

Microtubule-associated protein 7, MAP-7, Epithelial microtubule-associated protein of 115 kDa, Ensconsin, E-MAP-115

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  WB analysis of A549 using 13446-1-AP (same clone as 13446-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 13446-1-AP (same clone as 13446-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

  IP result of anti-MAP7 (IP:13446-1-AP, 4ug; Detection:13446-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 13446-1-AP (same clone as 13446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13446-1-AP (MAP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MAP7 antibody (13446-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13446-1-AP (same clone as 13446-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using MAP7 antibody 13446-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

  Immunofluorescent analysis of Hela cells, using MAP7 antibody 13446-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13446-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MAP7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAP7 fusion protein Ag4276
• Vollständiger Name: microtubule-associated protein 7
• Berechnetes Molekulargewicht: 749 aa, 84 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC025777
• Gene symbol: MAP7
• Gene ID (NCBI): 9053
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.