MAP7D1 Rekombinanter Antikörper

MAP7D1 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230424A6

Kat-Nr. : 83173-1-PBS

Synonyme

230424A6, Arginine/proline-rich coiled-coil domain-containing protein 1, KIAA1187, MAP7 domain-containing protein 1, PARCC1

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  WB analysis using 83173-1-RR (same clone as 83173-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83173-1-RR (MAP7D1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83173-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 83173-1-RR (same clone as 83173-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MAP7D1 antibody (83173-1-RR, Clone: 230424A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83173-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00212-2

  Cytometric bead array standard curve of MP00212-2, MAP7D1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83173-1-PBS. Detection antibody: 83173-3-PBS. Standard: Ag14385. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83173-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83173-1-RR against Human MAP7D1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83173-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA MAP7D1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MAP7D1 fusion protein Ag14385
• Vollständiger Name: MAP7 domain containing 1
• Berechnetes Molekulargewicht: 841 aa, 93 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC003083
• Gene symbol: MAP7D1
• Gene ID (NCBI): 55700
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP7D1 also known as RPRC1, PARCC1, belongs to the MAP7 family. The MAP7 (Microtubule Associated Protein 7) protein family, consisting of four members, MAP7, MAP7D1, and MAP7D2, MAP7D3, is the microtubule-associated protein involved in various cellular processes regulating microtubule dynamics, organization, and stability(PMID: 28980356). MAP7D1 exhibits the highest conservation with MAP7 and was recently identified as a phosphorylation substrate of DCLK1 in cortical neurons. MAP7D1 is required to maintain MT acetylation, which is enriched in stable MTs(PMID: 35470240). Consistent with the literature, the apparent molecular mass of MAP7D1 detected by Western blot was 120-130 kDa (PMID: 35470240, 37550720).