MAP7D1 Rekombinanter Antikörper

MAP7D1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230424A6

Kat-Nr. : 83173-1-RR

Synonyme

PP2464, PARCC1, MAP7 domain-containing protein 1, KIAA1187, Arginine/proline-rich coiled-coil domain-containing protein 1

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  WB analysis using 83173-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83173-1-RR (MAP7D1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 83173-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MAP7D1 antibody (83173-1-RR, Clone: 230424A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83173-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83173-1-RR against Human MAP7D1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MDA-MB-231-Zellen
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83173-1-RR bindet in WB, IF/ICC, ELISA MAP7D1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MAP7D1 fusion protein Ag14385
• Vollständiger Name: MAP7 domain containing 1
• Berechnetes Molekulargewicht: 841 aa, 93 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC003083
• Gene symbol: MAP7D1
• Gene ID (NCBI): 55700
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP7D1 also known as RPRC1, PARCC1, belongs to the MAP7 family. The MAP7 (Microtubule Associated Protein 7) protein family, consisting of four members, MAP7, MAP7D1, and MAP7D2, MAP7D3, is the microtubule-associated protein involved in various cellular processes regulating microtubule dynamics, organization, and stability(PMID: 28980356). MAP7D1 exhibits the highest conservation with MAP7 and was recently identified as a phosphorylation substrate of DCLK1 in cortical neurons. MAP7D1 is required to maintain MT acetylation, which is enriched in stable MTs(PMID: 35470240). Consistent with the literature, the apparent molecular mass of MAP7D1 detected by Western blot was 120-130 kDa (PMID: 35470240, 37550720).