JNK Polyklonaler Antikörper

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• KD/KO Validated

JNK Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(31)

Kat-Nr. : 17572-1-AP

Synonyme

c Jun N terminal kinase 3, JNK, JNK3, JNK3A, MAP kinase 10, MAP kinase p49 3F12, MAPK 10, MAPK10, p493F12, p54bSAPK, PRKM10

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  WB analysis using 17572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17572-1-AP (MAPK10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17572-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17572-1-AP (MAPK10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17572-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17572-1-AP (MAPK10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17572-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using JNK antibody (17572-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 31 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

17572-1-AP bindet in WB, IHC, IF/ICC, ELISA JNK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JNK fusion protein Ag11785
• Vollständiger Name: mitogen-activated protein kinase 10
• Berechnetes Molekulargewicht: 464 aa, 53 kDa
• Beobachtetes Molekulargewicht: 45-48 kDa, 54-57 kDa
• GenBank-Zugangsnummer: BC065516
• Gene symbol: JNK3
• Gene ID (NCBI): 5602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

JNK, also named MAPK10 (mitogen-activated protein kinase 10), belongs to MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is mainly expressed in a subset of neurons in the nervous system, and is activated by threonine and tyrosine phosphorylation. JNK has 3 isoforms of 48-53 kDa.

Publikationen

Species	Application	Title
	WB	Nat Commun; Protective effects of Pt-N-C single-atom nanozymes against myocardial ischemia-reperfusion injury; Authors - Tianbao Ye
rat	WB	J Neuroinflammation; SPOCK2 modulates neuropathic pain by interacting with MT1-MMP to regulate astrocytic MMP-2 activation in rats with chronic constriction injury; Authors - Chenglong Wang
mouse	WB	Int J Mol Sci; Sasanquasaponin from Camellia oleifera Abel Exerts an Anti-Inflammatory Effect in RAW 264.7 Cells via Inhibition of the NF-κB/MAPK Signaling Pathways; Authors - Yaxin Zhao
human,mouse	WB	Chem Biol Interact; Pulsatilla saponin D inhibited the growth of osteosarcoma by regulating the JNK/ATF3 signaling pathway; Authors - Kaipeng Jin
human	WB	Biomed Pharmacother; Icariside II suppresses cervical cancer cell migration through JNK modulated matrix metalloproteinase-2/9 inhibition in vitro and in vivo.; Authors - Ya-Sai Sun
human	WB,IHC	Onco Targets Ther; miR-137-3p Modulates the Progression of Prostate Cancer by Regulating the JNK3/EZH2 Axis.; Authors - Yachen Zang ; KD Validated