JNK Rekombinanter Antikörper

JNK Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242364A10

Kat-Nr. : 84875-5-PBS

Synonyme

MAPK10, 242364A10, c-Jun N-terminal kinase 3, EC:2.7.11.24, JNK1

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  IHC staining of mouse brain using 84875-5-RR (same clone as 84875-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84875-5-RR (MAPK10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84875-5-RR (same clone as 84875-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84875-5-RR (MAPK10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84875-5-RR (same clone as 84875-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using JNK antibody (84875-5-RR, Clone: 242364A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84875-5-RR (same clone as 84875-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAPK10 Recombinant antibody (84875-5-RR, Clone:242364A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84875-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84875-5-RR against Human MAPK10 were performed. The affinity constant is 2.45 nM.

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Geprüfte Anwendungen

Produktinformation

84875-5-PBS bindet in IHC, IF/ICC, FC (Intra), Indirect ELISA JNK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: JNK fusion protein Ag11785
• Vollständiger Name: mitogen-activated protein kinase 10
• Berechnetes Molekulargewicht: 464 aa, 53 kDa
• GenBank-Zugangsnummer: BC065516
• Gene symbol: JNK3
• Gene ID (NCBI): 5602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.