MAPK11 Polyklonaler Antikörper

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• KD/KO Validated

MAPK11 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17376-1-AP

Synonyme

MAP kinase 11, MAP kinase p38 beta, MAPK 11, MAPK11, p38 2, P38B, p38Beta, P38BETA2, PRKM11, SAPK2, SAPK2B

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Galerie der Validierungsdaten

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  WB analysis using 17376-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17376-1-AP (MAPK11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 17376-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17376-1-AP (MAPK11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17376-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17376-1-AP (MAPK11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 17376-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17376-1-AP (MAPK11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 17376-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17376-1-AP (MAPK11 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HEK-293 using 17376-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 17376-1-AP (MAPK11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Herzgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

17376-1-AP bindet in WB, IHC, IF, ELISA MAPK11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAPK11 fusion protein Ag11184
• Vollständiger Name: mitogen-activated protein kinase 11
• Berechnetes Molekulargewicht: 364 aa, 41 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC027933
• Gene symbol: MAPK11
• Gene ID (NCBI): 5600
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAPK11(Mitogen-activated protein kinase 11) is also named as PRKM11, SAPK2, SAPK2B,p38b and belongs to the MAP kinase subfamily.It is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors.In mouse brain, both p38a and p38b are present in neurons, while p38b is also expressed in glial cells(PMID:15748168).

Publikationen

Species	Application	Title
mouse	WB	Theranostics; Interleukin 33-mediated inhibition of A-type K+ channels induces sensory neuronal hyperexcitability and nociceptive behaviors in mice.; Authors - Yiru Wang
human	WB	Mol Pharmacol; p38β MAPK signaling mediates exenatide-stimulated microglial β-endorphin expression.; Authors - Hai-Yun Wu ; KD Validated
human	WB,IHC	Tumour Biol; Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.; Authors - Shutao Zheng ; KD Validated
rat	WB	BMC Endocr Disord; The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.; Authors - Shuman Wang ; KD Validated
	WB	J Hazard Mater; Metastatic effects of environmental carcinogens mediated by MAPK and UPR pathways with an in vivo Drosophila Model; Authors - Fangnon Firmin Fangninou