SAPK4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SAPK4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 10217-1-AP

Synonyme

MAP kinase 13, MAP kinase p38 delta, MAPK 13, MAPK13, p38delta, PRKM13, SAPK4

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  WB analysis using 10217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10217-1-AP (SAPK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10217-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10217-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

10217-1-AP bindet in WB, IHC, ELISA SAPK4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAPK4 fusion protein Ag0255
• Vollständiger Name: mitogen-activated protein kinase 13
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC000433
• Gene symbol: MAPK13
• Gene ID (NCBI): 5603
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
hamster	WB	Sci Rep; Cellular response to persistent foot-and-mouth disease virus infection is linked to specific types of alterations in the host cell transcriptome.; Authors - Lingling Han
human	WB,IHC	Tumour Biol; Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.; Authors - Shutao Zheng ; KD Validated
rat	WB	BMC Endocr Disord; The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.; Authors - Shuman Wang
mouse	WB	Nat Microbiol; ERK pathway reactivation prevents anthrax toxin lethality in mice; Authors - Jie Liu

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