JNK Polyklonaler Antikörper

JNK Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 17337-1-AP

Synonyme

MAPK9, JNK2A, JNK2, JNK 55, EC:2.7.11.24

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  WB analysis of mouse brain using 17337-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17337-1-AP (JNK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 17337-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 17337-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 1 publications below

Produktinformation

17337-1-AP bindet in WB, IF/ICC, ELISA JNK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JNK fusion protein Ag11372
• Vollständiger Name: mitogen-activated protein kinase 9
• Berechnetes Molekulargewicht: 424 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC032539
• Gene symbol: JNK
• Gene ID (NCBI): 5601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.

Publikationen

Species	Application	Title
human	WB	Biomed Pharmacother; Molecular mechanism through which Tripterygium hypoglaucum (Lévl.) Hutch alleviates psoriasis; Authors - Yumei Sun
mouse	WB	Int Immunopharmacol; RG108 attenuates acute kidney injury by inhibiting P38 MAPK/FOS and JNK/JUN pathways; Authors - Fan-Xu Kong
mouse	WB	Adv Sci (Weinh); Ultra-Long-Term Anti-Inflammatory Polyphenol Capsule to Remodel the Microenvironment for Accelerating Osteoarthritis Healing by Single Dosage; Authors - Shaoyin Wei
human	WB	BMC Cancer; Aldo-keto reductase family 1 member C3 mediates radioresistance of esophageal cancer cells through suppressing MAPK and AKT signaling; Authors - Wei Xiong
mouse	WB	Cardiovasc Diabetol; ANXA1-FPR2 axis mitigates the susceptibility to atrial fibrillation in obesity via rescuing AMPK activity in response to lipid overload; Authors - Peng Liu
rat	WB	Int Immunopharmacol; Kaempferol attenuates experimental autoimmune neuritis through TNFR1/JNK/p38 signaling pathway inhibition; Authors - Li Wang