MAPKAPK5 Polyklonaler Antikörper

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• KD/KO Validated

MAPKAPK5 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 14339-1-AP

Synonyme

MAPKAP-K5, MAPKAPK 5, MAPKAP kinase 5, MAPK-activated protein kinase 5, MAP kinase-activated protein kinase 5

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  WB analysis of HeLa using 14339-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14339-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 14339-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

14339-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MAPKAPK5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAPKAPK5 fusion protein Ag5650
• Vollständiger Name: mitogen-activated protein kinase-activated protein kinase 5
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC047284
• Gene symbol: MAPKAPK5
• Gene ID (NCBI): 8550
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAPKAPK5(MAP kinase-activated protein kinase 5) is also named as PRAK and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK5 contains the conserved protein kinase domains I through XI, which are characteristic of all protein kinases. It is a major stress-activated kinase that can phosphorylate small heat shock protein, suggesting a potential role for PRAK in mediating stress-induced HSP27 phosphorylation in vivo(PMID:9628874). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Nat Commun; The essential role of PRAK in tumor metastasis and its therapeutic potential.; Authors - Yuqing Wang ; KO Validated
human	WB, IP	J Cancer Biol Res; Cross-Phosphorylation and Interaction between Src/FAK and MAPKAP5/PRAK in Early Focal Adhesions Controls Cell Motility.; Authors - Sheila Figel Dwyer

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

hala (Verified Customer) (06-16-2022)

it works very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepG2