MAPKSP1 Polyklonaler Antikörper

MAPKSP1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 11937-1-AP

Synonyme

LAMTOR3, MAP2K1IP1, MAPBP, MAPK scaffold protein 1, MAPKSP1, MEK binding partner 1, MP1

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  WB analysis of human liver using 11937-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11937-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11937-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 11937-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11937-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11937-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 11937-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 11937-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 11937-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 11937-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11937-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, L02-Zellen, Maushirngewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Herzgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

11937-1-AP bindet in WB, IHC, ELISA MAPKSP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAPKSP1 fusion protein Ag2534
• Vollständiger Name: MAPK scaffold protein 1
• Berechnetes Molekulargewicht: 124 aa, 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC026245
• Gene symbol: MAPKSP1
• Gene ID (NCBI): 8649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAPKSP1, also named as mitogen activated protein kinase kinase 1 interacting protein 1, Map2k1ip1, MEK partner 1, Lamtor3 and MP1. It was first identified as a scaffold protein enhancing the efficiency of the MAPK pathway by facilitating the interaction between MEK1 and ERK1 upon serum stimulation (PMID: 9733512). Later, it has been demonstrated that MAPKSP1 enhances activation of both ERK1 and ERK2 as a response to EGF (PMID: 12479806) and fibronectin (PMID: 15923628). MAPKSP1 is involved in regulating endosomal signaling (PMID: 17178906), and a role in cancer, via MEK and ERK hyperactivation, has been demostrated in pancreatic tumorigenesis (PMID: 24209743). MAPKSP1 has two isoforms with the molecular weight of 13-14 kDa.

Publikationen

Species	Application	Title
human	WB	Front Pharmacol; Brucea javanica Oil Emulsion Promotes Autophagy in Ovarian Cancer Cells Through the miR-8485/LAMTOR3/mTOR/ATG13 Signaling Axis; Authors - Yihan Wang
human	WB	EMBO J; Micropeptide hSPAR regulates glutamine levels and suppresses mammary tumor growth via a TRIM21-P27KIP1-mTOR axis; Authors - Yan Huang