MAPRE2 Polyklonaler Antikörper

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MAPRE2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 10364-1-AP

Synonyme

APC binding protein EB2, APC-binding protein EB2, EB2, End-binding protein 2, Microtubule-associated protein RP/EB family member 2

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  WB analysis of K-562 using 10364-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10364-1-AP (MAPRE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10364-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10364-1-AP (MAPRE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 10364-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10364-1-AP (MAPRE2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cells using 10364-1-AP

  Mouse keratinocyte showing MAPRE2 (#10364-1-AP, 1:100 dilution, with Alexa 594 labelled 2nd Ab) in red and alpha-tubulin (#66031-1-lg, 1:100 dilution, with Alexa 488 labelled 2nd Ab ) in green.

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  IF Staining of HCT 116 using 10364-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using MAPRE2 antibody (10364-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HCT 116-Zellen, Mauszellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

10364-1-AP bindet in WB, IHC, IF/ICC, ELISA MAPRE2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAPRE2 fusion protein Ag0416
• Vollständiger Name: microtubule-associated protein, RP/EB family, member 2
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 39-42 kDa
• GenBank-Zugangsnummer: BC007318
• Gene symbol: MAPRE2
• Gene ID (NCBI): 10982
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RP1 protein shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family and binds specifically to the C-terminal region of the APC protein. RP1 also binds directly to tubulin, both in vitro and in vivo. The function of RP1 is currently unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. It may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR.

Publikationen

Species	Application	Title
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
mouse	WB	Exp Cell Res; MAPRE2 regulates the first meiotic progression in mouse oocytes.; Authors - Yuan-Yuan Li ; KD Validated

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