TAU Polyklonaler Antikörper

TAU Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

38

Rezensionen (2)

Kat-Nr. : 10274-1-AP

Synonyme

DDPAC, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, PPND, TAU

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 10274-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10274-1-AP (TAU antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 10274-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 10274-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human gliomas using 10274-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10274-1-AP (TAU antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10274-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10274-1-AP (TAU antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 10274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, rat brain
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 29 publications below
• IHC: See 3 publications below
• IF: See 8 publications below

Produktinformation

10274-1-AP bindet in WB, IHC, IF, ELISA TAU und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAU fusion protein Ag0354
• Vollständiger Name: microtubule-associated protein tau
• Berechnetes Molekulargewicht: 37-46, 79-81 kDa
• Beobachtetes Molekulargewicht: 45-70 kDa, 100 kDa
• GenBank-Zugangsnummer: BC000558
• Gene symbol: MAPT
• Gene ID (NCBI): 4137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Present polyclonal anti-Tau antibody was produced by immunizing animals with N-terminal of Tau and can detect approx 45-70 kDa and 100-kDa bands in brain tissues.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.; Authors - Xinwen Zhang
human		Brain; Tau accelerates α-synuclein aggregation and spreading in Parkinson's disease.; Authors - Lina Pan
mouse	IHC	Hum Mol Genet; Parkin deletion causes cerebral and systemic amyloidosis in human mutated tau over-expressing mice.; Authors - Rodríguez-Navarro Jose A JA
rat	WB	Acta Neuropathol Commun; Laterality and region-specific tau phosphorylation correlate with PTSD-related behavioral traits in rats exposed to repetitive low-level blast.; Authors - Georgina Perez Garcia
mouse,rat	WB	Free Radic Biol Med; The neuroprotective effects of phosphocreatine on amyloid beta 25-35-induced differentiated neuronal cell death through inhibition of AKT/GSK-3β /Tau/APP/CDK5 pathways in vivo and vitro; Authors - Jie Ai
mouse	IF	Mol Ther Nucleic Acids; Implications of miR-148a-3p/p35/PTEN signaling in tau hyperphosphorylation and autoregulatory feedforward of Akt/CREB in Alzheimer's disease.; Authors - Li Zeng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

David (Verified Customer) (01-02-2024)

Produces a single band at around 45kDa which is concordant with the data sheet.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y lysate

FH

Tanusree (Verified Customer) (02-14-2019)

The antibody worked well with the mouse primary cells and tissue.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse brain

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