MARCH1 Rekombinanter Antikörper

MARCH1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242143G10

Kat-Nr. : 85308-2-RR

Synonyme

MARCHF1, E3 ubiquitin-protein ligase MARCHF1, EC:2.3.2.27, MARCH I, MARCH-I

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  WB analysis of Daudi using 85308-2-RR

  Daudi cells were subjected to SDS PAGE followed by western blot with 85308-2-RR (MARCH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85308-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85308-2-RR (MARCH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 85308-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85308-2-RR (MARCH1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEL using 85308-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HEL cells using MARCH1 antibody (85308-2-RR, Clone: 242143G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85308-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85308-2-RR against Human MARCH1 were performed. The affinity constant is 29.7 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Daudi-Zellen, humanes Skelettmuskelgewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEL cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85308-2-RR bindet in WB, IHC, IF/ICC, ELISA MARCH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MARCH1 fusion protein Ag20231
• Vollständiger Name: membrane-associated ring finger (C3HC4) 1
• Berechnetes Molekulargewicht: 289 aa, 32 kDa
• Beobachtetes Molekulargewicht: 31-35 kDa
• GenBank-Zugangsnummer: BC153124
• Gene symbol: MARCHF1
• Gene ID (NCBI): 55016
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARCH1 is mainly found in secondary lymphoid organs, more specifically in the endocytic pathway of dendritic cells (DCs) and B cells (11-15). MARCH1 reduces the half-life of peptide/MHC II complexes by causing their redistribution from recycling endosomes to lysosomes. MARCH1 homodimerizes and also forms heterodimers with others family members (PMID: 22508929).