MARCH5 Polyklonaler Antikörper

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• KD/KO Validated

MARCH5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 12213-1-AP

Synonyme

MARCH V, MARCH5, Mitochondrial ubiquitin ligase, MITOL, RING finger protein 153, RNF153

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  WB analysis of HeLa using 12213-1-AP

  WB result of MARCH5 antibody (12213-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-MARCH5 transfected HeLa cells.

• 

  WB analysis using 12213-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12213-1-AP (MARCH5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 12213-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12213-1-AP (MARCH5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 12213-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12213-1-AP (MARCH5 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12213-1-AP bindet in WB, IHC, ELISA MARCH5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MARCH5 fusion protein Ag2806
• Vollständiger Name: membrane-associated ring finger (C3HC4) 5
• Berechnetes Molekulargewicht: 278 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa, 65-70 kDa
• GenBank-Zugangsnummer: BC015480
• Gene symbol: MARCH5
• Gene ID (NCBI): 54708
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARCH5(E3 ubiquitin-protein ligase MARCH5) is a 31 kDa protein also named as RNF153. This protein plays a protective role against mitochondrial dysfunction caused by the mitochondrial accumulation of mSOD1 via the ubiquitin-proteasome pathway (PMID:19741096). And it may regulate MAP1B LC1 function in mitochondria by controlling mitochondrial aggregation via mitochondrial LC1 levels(PMID:22308378). MEARCH5 can exsit as a homodimer with MW 65-70 kDa.

Publikationen

Species	Application	Title
human	WB	Clin Transl Med; FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression.; Authors - Lei Miao
mouse	WB	Biochim Biophys Acta Mol Cell Res; MARCH5 ameliorates aortic valve calcification via RACGAP1-DRP1 pathway associated mitochondrial quality control; Authors - Jialiang Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (04-09-2021)

HEK293 whole cell lysates ran on denaturing acrylamide gel and trasnferred to nitrocellulose membrane. Incubated overnight with antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293

MARCH5 Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:12213-1-AP)