MARCH5 Polyklonaler Antikörper

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• KD/KO Validated

MARCH5 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12213-1-PBS

Synonyme

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  WB analysis of HeLa using 12213-1-AP (same clone as 12213-1-PBS)

  WB result of MARCH5 antibody (12213-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-MARCH5 transfected HeLa cells. This data was developed using the same antibody clone with 12213-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12213-1-AP (same clone as 12213-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12213-1-AP (MARCH5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 12213-1-AP (same clone as 12213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12213-1-AP (MARCH5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 12213-1-AP (same clone as 12213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12213-1-AP (MARCH5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12213-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12213-1-PBS bindet in WB, IHC, Indirect ELISA MARCH5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MARCH5 fusion protein Ag2806
• Vollständiger Name: membrane-associated ring finger (C3HC4) 5
• Berechnetes Molekulargewicht: 278 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa, 65-70 kDa
• GenBank-Zugangsnummer: BC015480
• Gene symbol: MARCH5
• Gene ID (NCBI): 54708
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARCH5(E3 ubiquitin-protein ligase MARCH5) is a 31 kDa protein also named as RNF153. This protein plays a protective role against mitochondrial dysfunction caused by the mitochondrial accumulation of mSOD1 via the ubiquitin-proteasome pathway (PMID:19741096). And it may regulate MAP1B LC1 function in mitochondria by controlling mitochondrial aggregation via mitochondrial LC1 levels(PMID:22308378). MEARCH5 can exsit as a homodimer with MW 65-70 kDa.