MARCH8 Polyklonaler Antikörper

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• KD/KO Validated

MARCH8 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 14119-1-AP

Synonyme

c MIR, MARCH VIII, MARCH8, MIR, RING finger protein 178, RNF178

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 14119-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14119-1-AP (MARCH8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 14119-1-AP

  IP result of anti-MARCH8(IP:14119-1-AP, 4ug; Detection:14119-1-AP 1:500) with mouse testis tissue lysate 1120 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14119-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA MARCH8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MARCH8 fusion protein Ag5268
• Vollständiger Name: membrane-associated ring finger (C3HC4) 8
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC066988
• Gene symbol: MARCH8
• Gene ID (NCBI): 220972
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARCH8 (Membrane-associated RING finger protein 8) is also named as MIR, RNF178 and belongs to the RING zinc finger protein family. It functions as an E3 ubiquitin ligase for immune recognition-related molecules (e.g. major histocompatibility complex class I, B7-2, and ICAM-1). It plays a key role in controlling MHC class II surface expression by regulated ubiquitination of a lysine residue in the beta-chain (PMID:22761441).

Publikationen

Species	Application	Title
human	WB	Mol Cell; PMID: 28965816; Authors - Shouheng Jin ; KO Validated
human	WB	Nat Commun; MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.; Authors - Xiaoman Liu
		Autophagy; BST2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus nucleocapsid protein with selective autophagy.; Authors - Ning Kong ; KD Validated
human	WB	Autophagy; 2AB protein of Senecavirus A antagonizes selective autophagy and type I interferon production by degrading LC3 and MARCHF8.; Authors - Dage Sun ; KD Validated
human	WB	Proc Natl Acad Sci U S A; The E3 ubiquitin ligase MARCH8 negatively regulates IL-1β-induced NF-κB activation by targeting the IL1RAP coreceptor for ubiquitination and degradation.; Authors - Chen Rui R
human	WB	Cancers (Basel); MARCH8 Suppresses Tumor Metastasis and Mediates Degradation of STAT3 and CD44 in Breast Cancer Cells.; Authors - Wenjing Chen