MARCKS Polyklonaler Antikörper

MARCKS Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 20661-1-AP

Synonyme

MARCS, MACS, 80K-L protein, 80KL, 80K L protein

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  WB analysis using 20661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20661-1-AP (MARCKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20661-1-AP (MARCKS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 20661-1-AP

  IP result of anti-MARCKS (IP:20661-1-AP, 4ug; Detection:20661-1-AP 1:300) with A431 cells lysate 1200ug.

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  IHC staining of human normal colon using 20661-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20661-1-AP (MARCKS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 20661-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MARCKS antibody (20661-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: human normal colon; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

20661-1-AP bindet in WB, IHC, IF-P, IP, ELISA MARCKS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myristoylated alanine-rich protein kinase C substrate
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: NM_002356
• Gene symbol: MARCKS
• Gene ID (NCBI): 4082
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARCKS, also named as MACS, PRKCSL and 80K-L protein, belongs to the MARCKS family. MARCKS is the most prominent cellular substrate for protein kinase C. It binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Publikationen

Species	Application	Title
rat	WB	J Biol Chem; Oncoprotein DJ-1 interacts with mTOR complexes to effect transcription factor Hif1α-dependent expression of collagen I (α2) during renal fibrosis.; Authors - Falguni Das
human	WB	J Proteomics; Proteomic profile of human dental follicle stem cells and apical papilla stem cells.; Authors - Tong Lei
human	IP	Int J Oncol; Myristoylated alanine-rich C-kinase substrate effector domain phosphorylation regulates the growth and radiation sensitization of glioblastoma.; Authors - Nicholas J Eustace
human	WB,IF	Front Med (Lausanne); Enhanced understanding of cinnamaldehyde's therapeutic potential in osteoarthritis through bioinformatics and mechanistic validation of its anti-apoptotic effect; Authors - Yueyang Sheng
human	WB	J Vet Med Sci; A peptide against the N-terminus of myristoylated alanine-rich C kinase substrate promotes neuronal differentiation in SH-SY5Y human neuroblastoma cells; Authors - Jannatul Ferdous
mouse	WB	bioRxiv; Sex specific disruptions in Protein Kinase Cγ signaling in a mouse model of Spinocerebellar Ataxia Type 14; Authors - Sarah A Wolfe