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  • KD/KO Validated

MARK2 Polyklonaler Antikörper

MARK2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 15492-1-AP

Synonyme

ELKL motif kinase 1, EMK 1, EMK1, MARK2, PAR 1, PAR1 homolog, Par1b



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, Rattenhirngewebe
Erfolgreiche IPRattenhirngewebe
Erfolgreiche Detektion in IHChumanes Prostatakarzinomgewebe, humanes Hirngewebe, Maushodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15492-1-AP bindet in WB, IHC, IF, IP, ELISA MARK2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MARK2 fusion protein Ag7884
Vollständiger Name MAP/microtubule affinity-regulating kinase 2
Berechnetes Molekulargewicht 88 kDa
Beobachtetes Molekulargewicht 77-90 kDa
GenBank-ZugangsnummerBC008771
Gene symbol MARK2
Gene ID (NCBI) 2011
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARK2 is also named as EMK1(ELKL motif kinase 1), Par1b and belongs to the CAMK Ser/Thr protein kinase family. Par1b/MARK2 plays a critical role in neuronal polarity in the process of axon specification from multiple candidate neurites using primary cultures of mammalian hippocampal neurons(PMID:20194617). It has 16 isoforms produced by alternative promoter usage and alternative splicing with the molecular mass of 77-90kDa and can exsit as a dimer(PMID:16803889).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for MARK2 antibody 15492-1-APProtokoll herunterladen
IHC protocol for MARK2 antibody 15492-1-APProtokoll herunterladenl
IP protocol for MARK2 antibody 15492-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Mol Cell

An AMPK-Independent Signaling Pathway Downstream of the LKB1 Tumor Suppressor Controls Snail1 and Metastatic Potential.

Authors - Jonathan M Goodwin
humanIP

Nat Commun

TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth.

Authors - Tsz-Yin Chan
  • KD Validated
MouseWB,IHC

J Neurosci

Unbiased proteomics of early Lewy body formation model implicates active microtubule affinity-regulating kinases (MARKs) in synucleinopathies.

Authors - Michael X Henderson

FASEB J

Cdc42 is involved in NC1 peptide-regulated BTB dynamics through actin and microtubule cytoskeletal reorganization.

Authors - Wenhui Su
ratWB

FASEB J

Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane.

Authors - Ying Gao
humanWB

J Cell Sci

Molecular determinants of αVβ5 localization in flat clathrin lattices - role of αVβ5 in cell adhesion and proliferation.

Authors - Alba Zuidema
  • KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Lucas (Verified Customer) (08-28-2025)

The antibody is working reasonably in WB, but is not really suitable for IF, at least in the U251-MG cell line. This could be due to a large fraction of soluble MARK2 protein.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:250 (WB), 1:100(IF)
  • Cell Tissue Type: U251-MG