MARK2 Polyklonaler Antikörper

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• KD/KO Validated

MARK2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Rezensionen (1)

Kat-Nr. : 15492-1-AP

Synonyme

ELKL motif kinase 1, EMK 1, EMK1, MARK2, PAR 1, PAR1 homolog, Par1b

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  WB analysis of mouse brain using 15492-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15492-1-AP (MARK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15492-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15492-1-AP (MARK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15492-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15492-1-AP (MARK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 15492-1-AP

  IP result of anti-MARK2 (IP:15492-1-AP, 4ug; Detection:15492-1-AP 1:1000) with rat brain tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15492-1-AP (MARK2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15492-1-AP (MARK2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15492-1-AP (MARK2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15492-1-AP (MARK2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse testis using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15492-1-AP (MARK2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15492-1-AP (MARK2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Hirngewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

15492-1-AP bindet in WB, IHC, IF, IP, ELISA MARK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MARK2 fusion protein Ag7884
• Vollständiger Name: MAP/microtubule affinity-regulating kinase 2
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 77-90 kDa
• GenBank-Zugangsnummer: BC008771
• Gene symbol: MARK2
• Gene ID (NCBI): 2011
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARK2 is also named as EMK1(ELKL motif kinase 1), Par1b and belongs to the CAMK Ser/Thr protein kinase family. Par1b/MARK2 plays a critical role in neuronal polarity in the process of axon specification from multiple candidate neurites using primary cultures of mammalian hippocampal neurons(PMID:20194617). It has 16 isoforms produced by alternative promoter usage and alternative splicing with the molecular mass of 77-90kDa and can exsit as a dimer(PMID:16803889).

Publikationen

Species	Application	Title
human	WB	Mol Cell; An AMPK-Independent Signaling Pathway Downstream of the LKB1 Tumor Suppressor Controls Snail1 and Metastatic Potential.; Authors - Jonathan M Goodwin
human	IP	Nat Commun; TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth.; Authors - Tsz-Yin Chan ; KD Validated
Mouse	WB,IHC	J Neurosci; Unbiased proteomics of early Lewy body formation model implicates active microtubule affinity-regulating kinases (MARKs) in synucleinopathies.; Authors - Michael X Henderson
		FASEB J; Cdc42 is involved in NC1 peptide-regulated BTB dynamics through actin and microtubule cytoskeletal reorganization.; Authors - Wenhui Su
rat	WB	FASEB J; Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane.; Authors - Ying Gao
human	WB	J Cell Sci; Molecular determinants of αVβ5 localization in flat clathrin lattices - role of αVβ5 in cell adhesion and proliferation.; Authors - Alba Zuidema ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lucas (Verified Customer) (08-28-2025)

The antibody is working reasonably in WB, but is not really suitable for IF, at least in the U251-MG cell line. This could be due to a large fraction of soluble MARK2 protein.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:250 (WB), 1:100(IF)
• Cell Tissue Type: U251-MG