MAST1 Polyklonaler Antikörper

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• KD/KO Validated

MAST1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 13305-1-AP

Synonyme

SAST170, SAST, Microtubule-associated serine/threonine-protein kinase 1, MAST 1, KIAA0973

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  WB analysis using 13305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13305-1-AP (MAST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13305-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13305-1-AP (MAST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13305-1-AP

  IP result of anti-MAST1 (IP:13305-1-AP, 4ug; Detection:13305-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

13305-1-AP bindet in WB, IHC, IF, IP, ELISA MAST1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAST1 fusion protein Ag4126
• Vollständiger Name: microtubule associated serine/threonine kinase 1
• Berechnetes Molekulargewicht: 1570 aa, 171 kDa
• Beobachtetes Molekulargewicht: 171 kDa
• GenBank-Zugangsnummer: BC027985
• Gene symbol: MAST1
• Gene ID (NCBI): 22983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAST1(microtubule associated serine/threonine kinase 1) is also named as KIAA0973, SAST(syntrophin-associated serine/threonine-protein kinase), SAST170 and belongs to the family of microtubule-associated serine/threonine kinases, which has a unique structural feature with a serine/threonine kinase domain and a postsynaptic density protein-95/discs large/zona occludens-1 domain(PMID:12614677). This protein plays a role in a broad range of neural functions and it appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins.

Publikationen

Species	Application	Title
mouse	WB	Neuron; Mutations in MAST1 Cause Mega-Corpus-Callosum Syndrome with Cerebellar Hypoplasia and Cortical Malformations.; Authors - Ratna Tripathy ; KO Validated
human	WB,IHC,IF,IP	Theranostics; CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells; Authors - Apoorvi Tyagi