MATR3 Monoklonaler Antikörper

MATR3 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3B12C7

Kat-Nr. : 68280-1-Ig

Synonyme

Matrin-3, matrin 3, KIAA0723

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  WB analysis using 68280-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 68280-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 68280-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MATR3 antibody (68280-1-Ig, Clone: 3B12C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68280-1-Ig bindet in WB, IF/ICC, ELISA MATR3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MATR3 fusion protein Ag31502
• Vollständiger Name: matrin 3
• Berechnetes Molekulargewicht: 847 aa, 95 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC015031
• Gene symbol: MATR3
• Gene ID (NCBI): 9782
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MATR3, known as KIAA0723, is an 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa protein as a result of PMID: 28977530.