MAVS; VISA Polyklonaler Antikörper

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• KD/KO Validated

MAVS; VISA Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(78)

Rezensionen (1)

Kat-Nr. : 14341-1-AP

Synonyme

MAVS, Cardif, IPS 1, IPS1, IPS-1

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  WB analysis using 14341-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14341-1-AP

  WB result of MAVS; VISA antibody (14341-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAVS; VISA transfected HeLa cells.

• 

  WB analysis using 14341-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 14341-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14341-1-AP

  IP result of anti-MAVS; VISA (IP:14341-1-AP, 3ug; Detection:14341-1-AP 1:1000) with HEK-293 cells lysate 1700ug.

• 

  IHC staining of human breast cancer using 14341-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14341-1-AP (MAVS; VISA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14341-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14341-1-AP (MAVS; VISA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14341-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14341-1-AP (MAVS; VISA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14341-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (14341-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HepG2-Zellen, HuH-7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 16 publications below
• WB: See 81 publications below
• IHC: See 2 publications below
• IF: See 11 publications below
• IP: See 7 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Produktinformation

14341-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MAVS; VISA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Ente, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAVS; VISA fusion protein Ag5655
• Vollständiger Name: mitochondrial antiviral signaling protein
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa, 70-75 kDa
• GenBank-Zugangsnummer: BC044952
• Gene symbol: MAVS
• Gene ID (NCBI): 57506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

Publikationen

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
		Immunity; Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.; Authors - Yujia Wang
	WB	Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB	Nat Commun; MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation.; Authors - Peipei Zhou
human	WB	EMBO J; CircPVT1 promotes ER-positive breast tumorigenesis and drug resistance by targeting ESR1 and MAVS; Authors - Jia Yi ; KD Validated
pig	WB	Autophagy; Induction of autophagy and suppression of type I IFN secretion by CSFV.; Authors - Baoming Xie

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Damien (Verified Customer) (09-03-2019)

No convince by this anti-MAVS antibody.In fact, in human cells MAVS protein is expressed in two differents forms : short-MAVS (55kDa) and full-length MAVS (72 kDa). So I have to observe two bands by WB. However, after using different conditions with this antibody according to the manufacturer's protocol I always observe a smear of protein, and one band at 72 kDa. The expected on around 55 kDa never appear. Note that my cells lysates were not degraded since the others proteins that I want to reveal were great.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa