MAVS; VISA Monoklonaler Antikörper

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MAVS; VISA Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A8E9

Kat-Nr. : 66911-1-PBS

Synonyme

MAVS, 1A8E9, Cardif, IPS 1, IPS1

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  WB analysis of A431 using 66911-1-Ig (same clone as 66911-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

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  WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66911-1-Ig (same clone as 66911-1-PBS)

  WB result of MAVS; VISA antibody (66911-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAVS; VISA transfected HeLa cells. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66911-1-Ig (same clone as 66911-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66911-1-Ig (same clone as 66911-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50509-3

  Cytometric bead array standard curve of MP50509-3, MAVS; VISA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66911-1-PBS. Detection antibody: 66911-4-PBS. Standard:Ag5949. Range: 0.391-50 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66911-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA MAVS; VISA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAVS; VISA fusion protein Ag5949
• Vollständiger Name: mitochondrial antiviral signaling protein
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa, 70-75 kDa
• GenBank-Zugangsnummer: BC044952
• Gene symbol: MAVS
• Gene ID (NCBI): 57506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.