MAX Polyklonaler Antikörper

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• KD/KO Validated

MAX Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 10426-1-AP

Synonyme

bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, MAX, MYC associated factor X, orf1, Protein max

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10426-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP (MAX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10426-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP (MAX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10426-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP (MAX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• ChIP: See 4 publications below

Produktinformation

10426-1-AP bindet in WB, IHC, IF, ChIP, ELISA MAX und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAX fusion protein Ag0680
• Vollständiger Name: MYC associated factor X
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC003525
• Gene symbol: MAX
• Gene ID (NCBI): 4149
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.

Publikationen

Species	Application	Title
mouse	WB	Cancer Cell; MAX Functions as a Tumor Suppressor and Rewires Metabolism in Small Cell Lung Cancer.; Authors - Arnaud Augert ; KO Validated
human	WB	Sci Adv; A MYC inhibitor selectively alters the MYC and MAX cistromes and modulates the epigenomic landscape to regulate target gene expression.; Authors - Austin G Holmes
human	WB	Nat Commun; PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.; Authors - Xianchun Lan
human	WB,ChIP	Elife; Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness.; Authors - Haritha Mathsyaraja
human	WB	Cell Commun Signal; Novel dual-targeting c-Myc inhibitor D347-2761 represses myeloma growth via blocking c-Myc/Max heterodimerization and disturbing its stability.; Authors - Ruosi Yao
human	chIP	J Biol Chem; Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1.; Authors - Jianyin Long