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MAX Rekombinanter Antikörper

MAX Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230215D9

Kat-Nr. : 82958-6-PBS

Synonyme

bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8

Filter:
  • Western Blot (WB) analysis of various lysates using MAX Recombinant monoclonal antibody (82958-6-RR)

    WB analysis using 82958-6-RR (same clone as 82958-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82958-6-RR (MAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82958-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using MAX Recombinant monoclonal antibody (82958-6-RR)

    IF Staining of PC-3 using 82958-6-RR (same clone as 82958-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using MAX antibody (82958-6-RR, Clone: 230215D9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82958-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82958-6-RR

    Affinity and Kinetic Characterization of 82958-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82958-6-RR against Human MAX were performed. The affinity constant is 0.257 nM.

Geprüfte Anwendungen

Produktinformation

82958-6-PBS bindet in WB, IF/ICC, Indirect ELISA MAX und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen MAX fusion protein Ag0680
Vollständiger Name MYC associated factor X
Berechnetes Molekulargewicht 18 kDa
Beobachtetes Molekulargewicht22 kDa
GenBank-ZugangsnummerBC003525
Gene symbol MAX
Gene ID (NCBI) 4149
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.