MAZ Polyklonaler Antikörper

MAZ Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 21068-1-AP

Synonyme

MAZI, Myc-associated zinc finger protein, Pur 1, PUR1, Pur-1

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  WB analysis of HeLa using 21068-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21068-1-AP (MAZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21068-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21068-1-AP (MAZ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21068-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21068-1-AP (MAZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 21068-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MAZ antibody (21068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

21068-1-AP bindet in WB, IHC, IF/ICC, ChIP, ELISA MAZ und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAZ fusion protein Ag15200
• Vollständiger Name: MYC-associated zinc finger protein (purine-binding transcription factor)
• Berechnetes Molekulargewicht: 477 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49-55 kDa
• GenBank-Zugangsnummer: BC041629
• Gene symbol: MAZ
• Gene ID (NCBI): 4150
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myc-associated zinc finger protein (MAZ) is a six zinc finger protein with a G-rich binding motif. MAZ is often found at genomic binding sites adjacent to CTCF, a protein which affects large-scale genome organization through its interaction with cohesin (PMID: 33558242). MAZ is widely expressed in most human tissues, such as heart, lung, brain, liver, placenta, testis, colon, peripheral blood leukocytes, skeletal muscle, thymus, pancreas, prostate, thyroid, and adrenal gland (PMID: 35098656). In cancer, MAZ has been proved to be highly expressed in malignant tumors, and to promote cancer progression and metastasis by regulating the transcription of downstream target genes through directly binding with target gene promoters or synergistic action with other transcription factors.

Publikationen

Species	Application	Title
human	WB	Oxid Med Cell Longev; TUG1/MAZ/FTH1 Axis Attenuates the Antiglioma Effect of Dihydroartemisinin by Inhibiting Ferroptosis; Authors - Hailong Gong
mouse	IF	Cell Rep; Multi-omics profiling visualizes dynamics of cardiac development and functions; Authors - Yayun Gu
human	WB	Genomics; PLOD1 promote proliferation and migration with glycolysis via the Wnt/β-catenin pathway in THCA; Authors - Wei Cong
human	WB	Alzheimers Res Ther; Uncovering the epigenetic regulatory clues of PRRT1 in Alzheimer's disease: a strategy integrating multi-omics analysis with explainable machine learning; Authors - Fang Wang
human	WB,IHC,ChIP	Commun Biol; Molecular mechanisms of MAZ targeting up-regulation of NDUFS3 expression to promote malignant progression in melanoma; Authors - Yu Feng
human	WB	Oncogene; YBX1 promotes 5-Fluorouracil resistance in gastric cancer via m5C-dependent ATG9A mRNA stabilization through autophagy; Authors - Hongxin Huang ; KD Validated