MAZ Rekombinanter Antikörper

MAZ Rekombinant Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D5

Kat-Nr. : 82852-3-PBS

Synonyme

1D5, MAZI, Myc-associated zinc finger protein, Pur 1, PUR1

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  IHC staining of human prostate cancer using 82852-3-RR (same clone as 82852-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82852-3-RR (MAZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82852-3-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 82852-3-RR (same clone as 82852-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82852-3-RR (MAZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82852-3-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 82852-3-RR (same clone as 82852-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MAZ antibody (82852-3-RR, Clone: 1D5 ) at dilution of 1:950 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82852-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82852-3-RR (same clone as 82852-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAZ antibody (82852-3-RR, Clone: 1D5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82852-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82852-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82852-3-RR against Human MAZ were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82852-3-PBS bindet in IHC, IF/ICC, ELISA MAZ und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MAZ fusion protein Ag15200
• Vollständiger Name: MYC-associated zinc finger protein (purine-binding transcription factor)
• Berechnetes Molekulargewicht: 477 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49-51 kDa
• GenBank-Zugangsnummer: BC041629
• Gene symbol: MAZ
• Gene ID (NCBI): 4150
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myc-associated zinc-finger protein (MAZ) is a transcription factor with dual roles in transcription initiation and termination. MAZ can activate the expression of a variety of genes such as the c-Myc, insulin, VEGF, and RAS gene family and the serotonin 1A receptor. MAZ plays a critical role in the progression of various cancers including breast, prostate, liver, and so on. Deregulation of MAZ expression is associated with the progression of pancreatic ductal adenocarcinoma (PDAC)(PMID: 29414775).