MAdCAM1 Monoklonaler Antikörper

MAdCAM1 Monoklonal Antikörper für IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A12E8

Kat-Nr. : 66594-1-PBS

Synonyme

hMAdCAM 1, MACAM1, MAdCAM 1, MAdCAM1

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  IHC staining of human colon using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:500 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using MAdCAM1 antibody (66594-1-Ig, Clone: 2A12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using MAdCAM1 antibody (66594-1-Ig, Clone: 2A12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66594-1-PBS bindet in IHC, IF-P, Indirect ELISA MAdCAM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAdCAM1 fusion protein Ag16184
• Vollständiger Name: mucosal vascular addressin cell adhesion molecule 1
• Berechnetes Molekulargewicht: 406 aa, 43 kDa
• GenBank-Zugangsnummer: BC142629
• Gene symbol: MADCAM1
• Gene ID (NCBI): 8174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.