MAdCAM1 Monoklonaler Antikörper

MAdCAM1 Monoklonal Antikörper für IHC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IHC

Konjugation

Biotin

CloneNo.

2A12E8

Kat-Nr. : Biotin-66594

Synonyme

hMAdCAM 1, MACAM1, MAdCAM 1, MADCAM1

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  IHC staining of human small intestine using Biotin-66594

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using Biotin-66594 (MAdCAM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using Biotin-66594

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using Biotin-66594 (MAdCAM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using Biotin-66594

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66594 (MAdCAM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using Biotin-66594

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66594 (MAdCAM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-66594 bindet in IHC MAdCAM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAdCAM1 fusion protein Ag16184
• Vollständiger Name: mucosal vascular addressin cell adhesion molecule 1
• Berechnetes Molekulargewicht: 406 aa, 43 kDa
• GenBank-Zugangsnummer: BC142629
• Gene symbol: MADCAM1
• Gene ID (NCBI): 8174
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.