MAdCAM1 Monoklonaler Antikörper

MAdCAM1 Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF-P

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2A12E8

Kat-Nr. : CL594-66594

Synonyme

hMAdCAM 1, MACAM1, MAdCAM 1, MADCAM1

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  IF Staining of human tonsillitis using CL594-66594

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 MAdCAM1 antibody (CL594-66594, Clone: 2A12E8 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66594 bindet in IF-P MAdCAM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAdCAM1 fusion protein Ag16184
• Vollständiger Name: mucosal vascular addressin cell adhesion molecule 1
• Berechnetes Molekulargewicht: 406 aa, 43 kDa
• GenBank-Zugangsnummer: BC142629
• Gene symbol: MADCAM1
• Gene ID (NCBI): 8174
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.